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ORM1 Antibody Pair

CSB-EAP05709
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetORM1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ORM1 Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.6ug/ml and the detection antibody at a concentration of 0.34ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID5004
  • Target name
    ORM1
  • Target description
    orosomucoid 1
  • Target synonyms
    AGP 1; AGP1; AGP-A; alpha-1-acid glycoprotein 1; epididymis secretory sperm binding protein Li 153w; HEL-S-153w; OMD 1; ORM
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02763
  • Protein Name
    Alpha-1-acid glycoprotein 1
  • Reactivity
    Bovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of ORM1 using anti-ORM1 antibody (PB9956). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORM1 antigen affinity purified polyclonal antibody (Catalog # PB9956) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ORM1 at approximately 40-47 kDa. The expected band size for ORM1 is at 23 kDa.
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