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ProductsBack/Ornithine Decarboxylase (ODC1) Mouse Monoclonal Antibody [Clone ID: OTI1G6]
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human ODC1 plasmid (RC206858, hODC1), mouse ODC1 plasmid (MR207353, mODC1) using anti-ODC1 antibody TA501286 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human ODC1 plasmid (RC206858, hODC1), mouse ODC1 plasmid (MR207353, mODC1) using anti-ODC1 antibody TA501286 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human ODC1 plasmid (RC206858, hODC1), mouse ODC1 plasmid (MR207353, mODC1) using anti-ODC1 antibody TA501286 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Ornithine Decarboxylase (ODC1) Mouse Monoclonal Antibody [Clone ID: OTI1G6]

CF501286
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetODC1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Ornithine Decarboxylase (ODC1) Mouse Monoclonal Antibody [Clone ID: OTI1G6]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1G6
  • Gene ID4953
  • Target name
    ODC1
  • Target description
    ornithine decarboxylase 1
  • Target synonyms
    BABS, NEDBA, NEDBIA, ODC, ornithine decarboxylase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP11926
  • Protein Name
    Ornithine decarboxylase
  • Scientific Description
    Carrier-free (BSA/glycerol-free) ODC1 mouse monoclonal antibody, clone OTI1G6 (formerly 1G6)
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of ODC1 using anti-ODC1 antibody (A03138-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human LNCAP whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ODC1 antigen affinity purified polyclonal antibody (Catalog # A03138-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ODC1 at approximately 51 kDa. The expected band size for ODC1 is at 51,55 kDa.
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