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Osteocalcin Antibody

LS-C17034
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, RadioImmunoAssay
Product group Antibodies
TargetBGLAP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Osteocalcin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in RIA, ELISA, Western Blot and Immunohistochemistry. ELISA: 100-1000 ug/ml. Coat plate with Osteocalcin at 5-10 ug/ml. Western Blot: 10 ug/ml. Immunohistochemistry: 10-15 ug/ml. Note: Assays require 1-2 mM CaCl2.. ELISA (100 - 1000 µg/ml), IHC (10 - 15 µg/ml), RIA, WB (10 µg/ml) Suitable for use in RIA, ELISA, Western Blot and Immunohistochemistry. ELISA: 100-1000 ug/ml. Coat plate with Osteocalcin at 5-10 ug/ml. Western Blot: 10 ug/ml. Immunohistochemistry: 10-15 ug/ml. Note: Assays require 1-2 mM CaCl2.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, RadioImmunoAssay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID632
  • Target name
    BGLAP
  • Target description
    bone gamma-carboxyglutamate protein
  • Target synonyms
    BGP; bone gamma-carboxyglutamate (gla) protein (osteocalcin); gamma-carboxyglutamic acid-containing protein; OC; OCN; osteocalcin
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Osteocalcin Polyclonal Antibody(bs-0470R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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