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ProductsBack/Oviductin (H-8) Alexa Fluor(r) 790

Oviductin (H-8) Alexa Fluor(r) 790

SC-377267AF790
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetOVGP1
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200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    Oviductin (H-8) Alexa Fluor(r) 790
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 790
  • Gene ID5016
  • Target name
    OVGP1
  • Target description
    oviductal glycoprotein 1
  • Target synonyms
    CHIT5, EGP, MUC9, OGP, oviduct-specific glycoprotein, estrogen-dependent oviduct protein, mucin 9, oviduct glycoprotein, oviductal glycoprotein 1, 120kDa, oviductin
  • Host
    Mouse
  • Reactivity
    Hamster, Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OVGP1 using anti-OVGP1 antibody (A07911-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse ovary tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OVGP1 antigen affinity purified polyclonal antibody (Catalog # A07911-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OVGP1 at approximately 75 kDa. The expected band size for OVGP1 is at 75 kDa.
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