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Western blot All lanes: OXCT1 antibody at 8ug/ml Lane 1: Mouse heart tissue Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 57, 14 kDa Observed band size: 57 kDa
Western blot All lanes: OXCT1 antibody at 8ug/ml Lane 1: Mouse heart tissue Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 57, 14 kDa Observed band size: 57 kDa
Western blot All lanes: OXCT1 antibody at 8ug/ml Lane 1: Mouse heart tissue Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 57, 14 kDa Observed band size: 57 kDa

OXCT1 Antibody

CSB-PA017306ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetOXCT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    OXCT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5019
  • Target name
    OXCT1
  • Target description
    3-oxoacid CoA-transferase 1
  • Target synonyms
    OXCT, SCOT, succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial, 3-oxoacid CoA transferase, SCOT-s, epididymis secretory sperm binding protein, somatic-type succinyl-CoA:3-oxoacid CoA-transferase, succinyl CoA:3-oxoacid CoA transferase, succinyl-CoA:3-ketoacid-CoA transferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55809
  • Protein Name
    Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial
  • Scientific Description
    Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of OXCT1/SCOT using anti-OXCT1/SCOT antibody (A07229-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: monkey heart tissue lysates, Lane 4: human placenta tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse heart tissue lysates, Lane 9: mouse kidney tissue lysates, Lane 10: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXCT1/SCOT antigen affinity purified polyclonal antibody (Catalog # A07229-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OXCT1/SCOT at approximately 56KD. The expected band size for OXCT1/SCOT is at 56KD.
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