Bio-Connect
WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX32770 OXCT1 antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX32770 OXCT1 antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX32770 OXCT1 antibody. Dilution : 1:3000 Loading : 25microg per lane

OXCT1 antibody

GTX32770
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetOXCT1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    OXCT1 antibody - KO/KD Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5019
  • Target name
    OXCT1
  • Target description
    3-oxoacid CoA-transferase 1
  • Target synonyms
    3-oxoacid CoA transferase; epididymis secretory sperm binding protein; OXCT; SCOT; SCOT-s; somatic-type succinyl-CoA:3-oxoacid CoA-transferase; succinyl CoA:3-oxoacid CoA transferase; succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial; succinyl-CoA:3-ketoacid-CoA transferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55809
  • Protein Name
    Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial
  • Scientific Description
    This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetOXCT1
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetOXCT1
  • SizePrice
Product group Antibodies
Assay Genie
TargetOXCT1
  • SizePrice
Western blot All lanes: OXCT1 antibody at 8ug/ml Lane 1: Mouse heart tissue Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 57, 14 kDa Observed band size: 57 kDa
Product group Antibodies
Cusabio
OXCT1 AntibodyCSB-PA017306ESR1HU
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetOXCT1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
OXCT1 AntibodyLS-C409675
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetOXCT1
  • SizePrice
Anti-OXCT1 Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot
  • SizePrice
Product group Antibodies
Bethyl Laboratories
ApplicationsWestern Blot
TargetOXCT1
  • SizePrice
Immunohistochemistry analysis in human heart muscle and liver tissues using HPA012047 antibody. Corresponding OXCT1 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetOXCT1
  • SizePrice
Figure 1. Western blot analysis of OXCT1/SCOT using anti-OXCT1/SCOT antibody (A07229-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: monkey heart tissue lysates, Lane 4: human placenta tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse heart tissue lysates, Lane 9: mouse kidney tissue lysates, Lane 10: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXCT1/SCOT antigen affinity purified polyclonal antibody (Catalog # A07229-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OXCT1/SCOT at approximately 56KD. The expected band size for OXCT1/SCOT is at 56KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetOXCT1
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded Human esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with OXCT1 Polyclonal Antibody, Unconjugated (bs-55159R) at 1:100 overnight at 4°C, DAB staining.
Product group Antibodies
Bioss Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetOXCT1
  • SizePrice