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ProductsBack/OXSR1 Mouse Monoclonal Antibody [Clone ID: OTI1F3]
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HepG2 cells(WT) and OXSR1-Knockdown HepG2 cells(KD) were separated by SDS-PAGE and immunoblotted with anti-OXSR1 monoclonal antibody TA500392(1:2000).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HepG2 cells(WT) and OXSR1-Knockdown HepG2 cells(KD) were separated by SDS-PAGE and immunoblotted with anti-OXSR1 monoclonal antibody TA500392(1:2000).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HepG2 cells(WT) and OXSR1-Knockdown HepG2 cells(KD) were separated by SDS-PAGE and immunoblotted with anti-OXSR1 monoclonal antibody TA500392(1:2000).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.

OXSR1 Mouse Monoclonal Antibody [Clone ID: OTI1F3]

CF500392
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetOXSR1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    OXSR1 Mouse Monoclonal Antibody [Clone ID: OTI1F3]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1F3
  • Gene ID9943
  • Target name
    OXSR1
  • Target description
    oxidative stress responsive kinase 1
  • Target synonyms
    OSR1, serine/threonine-protein kinase OSR1, oxidative stress responsive 1, oxidative stress-responsive 1 protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO95747
  • Protein Name
    Serine/threonine-protein kinase OSR1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) OXSR1 mouse monoclonal antibody, clone OTI1F3 (formerly 1F3)
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of OXSR1 using anti-OXSR1 antibody (A05141-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXSR1 antigen affinity purified polyclonal antibody (Catalog # A05141-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OXSR1 at approximately 58 kDa. The expected band size for OXSR1 is at 58 kDa.
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