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Phospho-OXSR1 (T185) Antibody

ORB571456
Biorbyt
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetOXSR1
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Phospho-OXSR1 (T185) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB: 1:500-1:2000, IHC-P: 1:100-1:300, ELISA: 1:10000 ELISA, IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9943
  • Target name
    OXSR1
  • Target description
    oxidative stress responsive kinase 1
  • Target synonyms
    OSR1, serine/threonine-protein kinase OSR1, oxidative stress responsive 1, oxidative stress-responsive 1 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95747
  • Protein Name
    Serine/threonine-protein kinase OSR1
  • Scientific Description
    Phospho-OXSR1 (T185) Antibody
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OXSR1 using anti-OXSR1 antibody (A05141-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXSR1 antigen affinity purified polyclonal antibody (Catalog # A05141-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OXSR1 at approximately 58 kDa. The expected band size for OXSR1 is at 58 kDa.
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