Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas antibody [GT331] (GTX633771) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
![p130Cas antibody [GT331] detects p130Cas protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. p130Cas antibody [GT331] (GTX633771) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX633771/GTX633771_42623_20170818_IHC-P_M_w_23061202_577.webp "p130Cas antibody [GT331] detects p130Cas protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. p130Cas antibody [GT331] (GTX633771) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![p130Cas antibody [GT331] detects p130Cas protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: p130Cas protein stained by p130Cas antibody [GT331] (GTX633771) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX633771/GTX633771_42623_20170831_IHC-Fr_M_w_23061202_373.webp "p130Cas antibody [GT331] detects p130Cas protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: p130Cas protein stained by p130Cas antibody [GT331] (GTX633771) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min")
![p130Cas antibody [GT331] detects p130Cas protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. p130Cas antibody [GT331] (GTX633771) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX633771/GTX633771_42623_20170818_IHC-P_R_w_23061202_923.webp "p130Cas antibody [GT331] detects p130Cas protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. p130Cas antibody [GT331] (GTX633771) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas antibody [GT331] (GTX633771) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
p130Cas antibody [GT331]
GTX633771
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBCAR1
Overview
- SupplierGeneTex
- Product Namep130Cas antibody [GT331]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT331
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID9564
- Target nameBCAR1
- Target descriptionBCAR1 scaffold protein, Cas family member
- Target synonymsCAS, CAS1, CASS1, CRKAS, P130Cas, breast cancer anti-estrogen resistance protein 1, BCAR1, Cas family scaffold protein, BCAR1, Cas family scaffolding protein, Cas scaffolding protein family member 1, Crk-associated substrate p130Cas
- HostMouse
- IsotypeIgG1
- Protein IDP56945
- Protein NameBreast cancer anti-estrogen resistance protein 1
- Scientific DescriptionBCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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