![FACS analysis of Jurkat cells using GTX22814 p23 antibody [JJ3] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde FACS analysis of Jurkat cells using GTX22814 p23 antibody [JJ3] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX22814/GTX22814_159_FACS_w_23060620_817.webp)
FACS analysis of Jurkat cells using GTX22814 p23 antibody [JJ3] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
p23 antibody [JJ3]
GTX22814
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityChicken, Guinea Pig, Human, Mouse, Rabbit, Rat
TargetPTGES3
Overview
- SupplierGeneTex
- Product Namep23 antibody [JJ3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1,000. ICC/IF: 1:200-1:2000. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDJJ3
- ConjugateUnconjugated
- Gene ID10728
- Target namePTGES3
- Target descriptionprostaglandin E synthase 3
- Target synonymsP23, TEBP, cPGES, prostaglandin E synthase 3, Hsp90 co-chaperone, cytosolic prostaglandin E synthase, cytosolic prostaglandin E2 synthase, progesterone receptor complex p23, prostaglandin E synthase 3 (cytosolic), telomerase-binding protein p23, unactive progesterone receptor, 23 kD
- HostMouse
- IsotypeIgG1
- Protein IDQ15185
- Protein NameProstaglandin E synthase 3
- Scientific DescriptionThis gene encodes an enzyme that converts prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). This protein functions as a co-chaperone with heat shock protein 90 (HSP90), localizing to response elements in DNA and disrupting transcriptional activation complexes. Alternative splicing results in multiple transcript variants. There are multiple pseudogenes of this gene on several different chromosomes. [provided by RefSeq, Feb 2016]
- ReactivityChicken, Guinea Pig, Human, Mouse, Rabbit, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![IHC-P analysis of human tonsil tissue using GTX22814 p23 antibody [JJ3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:1000 IHC-P analysis of human tonsil tissue using GTX22814 p23 antibody [JJ3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22814/GTX22814_1149_IHC-P_w_23060620_220.webp)
![ICC/IF analysis of HeLa cells using GTX22814 p23 antibody [JJ3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:500 overnight at 4oC ICC/IF analysis of HeLa cells using GTX22814 p23 antibody [JJ3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:500 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22814/GTX22814_497_ICC-IF_w_23060620_864.webp)
![IHC-P analysis of human testis tissue using GTX22814 p23 antibody [JJ3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:1000 IHC-P analysis of human testis tissue using GTX22814 p23 antibody [JJ3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22814/GTX22814_1148_IHC-P_w_23060620_145.webp)
![ICC/IF analysis of MCF-7 cells using GTX22814 p23 antibody [JJ3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:500 overnight at 4oC ICC/IF analysis of MCF-7 cells using GTX22814 p23 antibody [JJ3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:500 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22814/GTX22814_495_ICC-IF_w_23060620_613.webp)
![ICC/IF analysis of C6 cells using GTX22814 p23 antibody [JJ3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:500 overnight at 4oC ICC/IF analysis of C6 cells using GTX22814 p23 antibody [JJ3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:500 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22814/GTX22814_496_ICC-IF_w_23060620_406.webp)
![FACS analysis of 3T3 cells using GTX22814 p23 antibody [JJ3] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde FACS analysis of 3T3 cells using GTX22814 p23 antibody [JJ3] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX22814/GTX22814_157_FACS_w_23060620_195.webp)
![IHC-P analysis of human breast carcinoma tissue using GTX22814 p23 antibody [JJ3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:1000 IHC-P analysis of human breast carcinoma tissue using GTX22814 p23 antibody [JJ3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22814/GTX22814_1147_IHC-P_w_23060620_141.webp)
![FACS analysis of Hela cells using GTX22814 p23 antibody [JJ3] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde FACS analysis of Hela cells using GTX22814 p23 antibody [JJ3] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX22814/GTX22814_158_FACS_w_23060620_849.webp)
![WB analysis of 25 ug of Hela (Lane 1), HepG2 (Lane 2), and mouse spleen cell lysates (Lane 3) using GTX22814 p23 antibody [JJ3]. Dilution : 1:1000 WB analysis of 25 ug of Hela (Lane 1), HepG2 (Lane 2), and mouse spleen cell lysates (Lane 3) using GTX22814 p23 antibody [JJ3]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22814/GTX22814_1606_WB_w_23060620_402.webp)

![Wild-type (WT) and p23 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with p23 antibody [N1C3] (GTX106550) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX106550/GTX106550_40401_20180209_WB_KO_watermark_w_23060120_812.webp)




