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P2RX7 Antibody (YA4449, Mouse)

HY-P84752
MedChem Express
TargetP2RX7
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    P2RX7 Antibody (YA4449, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5027
  • Target name
    P2RX7
  • Target description
    purinergic receptor P2X 7
  • Target synonyms
    ATP receptor; P2X purinoceptor 7; P2X7; P2X7 receptor; P2Z receptor; purinergic receptor P2X, ligand gated ion channel, 7; purinergic receptor P2X7 variant A; purnergic receptor P2X 7
  • Host
    Mouse
  • Isotype
    IgG2b
  • Scientific Description
    P2RX7 Antibody (YA4449) is a mouse-derived and non-conjugated IgG2b antibody, targeting to P2RX7. It can be applicated for WB, IHC-P, FC, ELISA assays, in the background of human, mouse, rabbit.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Immunofluorescence staining of Hela cells with CSB-PA859103LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Figure 1. Western blot analysis of P2X7/P2RX7 using anti-P2X7/P2RX7 antibody (A01208-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2X7/P2RX7 antigen affinity purified polyclonal antibody (Catalog # A01208-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2X7/P2RX7 at approximately 70 kDa. The expected band size for P2X7/P2RX7 is at 70 kDa.
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