p53 antibody [Pab240]

GeneTex

p53 antibody [Pab240] - Orthogonal Validated

9

For IHC: Use at a dilution of 1:250 - 1:500. Extensive washing may be necessary. This product does not require protein digestion or microwave antigen retrieval prior to staining. For IP: Use at 10microg/mg of lysate. By immunoprecipitation, this antibody reacts with only MUTANT p53protein under NON-DENATURING conditions. For WB: Use at a dilution of 1:2,000. Predicted molecular weight: 53 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

Pab240

2 mg/ml

Unconjugated

TP53

tumor protein p53

BCC7, BMFS5, LFS1, P53, TRP53, cellular tumor antigen p53, antigen NY-CO-13, mutant tumor protein 53, phosphoprotein p53, transformation-related protein 53, tumor protein 53, tumor supressor p53

Mouse

IgG1

Cellular tumor antigen p53

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

-20°C or -80°C,2°C to 8°C

12352203