More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/P53 Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded rat bladder tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-p53 (FL-393) Polyclonal Antibody, Unconjugated (bs-8687R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat bladder tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-p53 (FL-393) Polyclonal Antibody, Unconjugated (bs-8687R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat bladder tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-p53 (FL-393) Polyclonal Antibody, Unconjugated (bs-8687R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining

P53 Polyclonal Antibody

BS-8687R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rat, Sheep
TargetTP53
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    P53 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7157
  • Target name
    TP53
  • Target description
    tumor protein p53
  • Target synonyms
    BCC7, BMFS5, LFS1, P53, TRP53, cellular tumor antigen p53, antigen NY-CO-13, mutant tumor protein 53, phosphoprotein p53, transformation-related protein 53, tumor protein 53, tumor supressor p53
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04637
  • Protein Name
    Cellular tumor antigen p53
  • Reactivity
    Bovine, Canine, Equine, Human, Mouse, Porcine, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Zhan C, Liu G, Li J, et al. Rotenone and 3-bromopyruvate toxicity impacts electrical and structural cardiac remodeling in rats. Toxicol Lett. 2020,318:57-64. doi: 10.1016/j.toxlet.2019.09.024
    Read this paper
  • Zhang W, Zhao Y, Zhang P, et al. Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable. Chemosphere. 2018,194:147-157. doi: 10.1016/j.chemosphere.2017.11.164
    Read this paper
  • Zeng K, Feng QG, Lin BT, et al. Effects of microRNA-211 on proliferation and apoptosis of lens epithelial cells by targeting SIRT1 gene in diabetic cataract mice. Biosci Rep. 2017,37(4):pii: BSR20170695. doi: 10.1042/BSR20170695.
    Read this paper
  • Qin Q, Xiong ZA, Liu Y, et al. Effects of irreversible electroporation on cervical cancer cell lines in vitro. Mol Med Rep. 2016,14(3):2187-93. doi: 10.3892/mmr.2016.5468
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Figure 1. Western blot analysis of TP53 using anti-TP53 antibody (A00001-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53 antigen affinity purified polyclonal antibody (Catalog # A00001-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53 at approximately 53 kDa. The expected band size for TP53 is at 44 kDa.
Product group Antibodies
Boster Bio
Anti-TP53 Antibody Picoband(r)A00001-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetTP53
  • SizePrice
Product group Antibodies
RayBiotech
Anti-P53 Antibody130-10102
ApplicationsELISA
  • SizePrice
Product group Antibodies
References
GeneTex
p53 antibody [N1], N-termGTX100629
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetTP53
  • SizePrice
Product group Antibodies
Assay Genie
p53 AntibodyCAB0263
ReactivityHuman
TargetTP53
  • SizePrice
Product group Antibodies
Biomatik
Tp53 Polyclonal AntibodyCAC07006
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetTP53
  • SizePrice
Immunohistochemistry analysis in human skin and skeletal muscle tissues using AMAb90956 antibody. Corresponding p53 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-p53 AntibodyAMAB90956
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTP53
  • SizePrice
Anti-p53 Antibody
Product group Antibodies
Antibodies.com
Anti-p53 AntibodyA101571
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
Product group Antibodies
Cusabio
TP53 Monoclonal AntibodyCSB-MA000208
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetTP53
  • SizePrice
Product group Antibodies
Applied Biological Materials
Anti-p53Y058191
ApplicationsWestern Blot, ELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-p53 [PAb421]Ab00142-2.0
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
ReactivityHuman, Monkey, Mouse, Rabbit, Rat
TargetTP53
  • SizePrice