ICC/IF analysis of HeLa cells treated with 100 nM Doxorubicin for 24 hrs using GTX00678 p53 (phospho Ser315) antibody [#18]. Dilution : 1:1000 in 1% BSA in PBS, overnight, 4 degree C Fixation : 4% PFA, overnight Permeabilization : 0.25% Triton X-100 in PBS for 10 min
![IHC-P analysis of Human stomach cancer tissue section using GTX00678 p53 (phospho Ser315) antibody [#18].](https://www.genetex.com/upload/website/prouct_img/normal/GTX00678/GTX00678_20191104_IHC-P_w_23053121_607.webp "IHC-P analysis of Human stomach cancer tissue section using GTX00678 p53 (phospho Ser315) antibody [#18].")
![WB analysis of various samples using GTX00678 p53 (phospho Ser315) antibody [#18] (upper) or GTX70214 p53 antibody [DO1] (lower). Lane 1 : untreated MCF cells Lane 2 : MCF cells treated with nocodazole at 100 ng/ml for 48 hrs](https://www.genetex.com/upload/website/prouct_img/normal/GTX00678/GTX00678_20191104_WB_w_23053121_646.webp "WB analysis of various samples using GTX00678 p53 (phospho Ser315) antibody [#18] (upper) or GTX70214 p53 antibody [DO1] (lower). Lane 1 : untreated MCF cells Lane 2 : MCF cells treated with nocodazole at 100 ng/ml for 48 hrs")
ICC/IF analysis of HeLa cells treated with 100 nM Doxorubicin for 24 hrs using GTX00678 p53 (phospho Ser315) antibody [#18]. Dilution : 1:1000 in 1% BSA in PBS, overnight, 4 degree C Fixation : 4% PFA, overnight Permeabilization : 0.25% Triton X-100 in PBS for 10 min
p53 (phospho Ser315) antibody [#18] - Orthogonal Validated
GTX00678
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTP53
Overview
- SupplierGeneTex
- Product Namep53 (phospho Ser315) antibody [#18] - Orthogonal Validated
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID#18
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7157
- Target nameTP53
- Target descriptiontumor protein p53
- Target synonymsantigen NY-CO-13; BCC7; BMFS5; cellular tumor antigen p53; LFS1; mutant tumor protein 53; P53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; TRP53; tumor protein 53; tumor supressor p53
- HostMouse
- IsotypeIgG2b
- Modification TypePhosphorylated
- Scientific DescriptionThis gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
- ReactivityHuman
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203
References
- Curcumin Rescues Doxorubicin Responsiveness via Regulating Aurora a Signaling Network in Breast Cancer Cells. Biswas S et al., 2021 Mar 1, Asian Pac J Cancer PrevRead more