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Formalin-fixed and paraffin embedded human ovarian carcinoma labeled with Rabbit Anti-P53 protein(wt-p53) Polyclonal Antibody (bs-0033R), Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human ovarian carcinoma labeled with Rabbit Anti-P53 protein(wt-p53) Polyclonal Antibody (bs-0033R), Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human ovarian carcinoma labeled with Rabbit Anti-P53 protein(wt-p53) Polyclonal Antibody (bs-0033R), Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining

P53 Polyclonal Antibody

BS-0033R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTP53
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Overview

  • Supplier
    Bioss
  • Product Name
    P53 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7157
  • Target name
    TP53
  • Target description
    tumor protein p53
  • Target synonyms
    BCC7, BMFS5, LFS1, P53, TRP53, cellular tumor antigen p53, antigen NY-CO-13, mutant tumor protein 53, phosphoprotein p53, transformation-related protein 53, tumor protein 53, tumor supressor p53
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04637
  • Protein Name
    Cellular tumor antigen p53
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Tan J, Qin X, Liu B, et al. Integrative findings indicate anti-tumor biotargets and molecular mechanisms of calycosin against osteosarcoma. Biomed Pharmacother. 2020,126:110096. doi: 10.1016/j.biopha.2020.110096
    Read this paper
  • Muhammad T, Sakhawat A, Khan AA, et al. Aloperine in combination with therapeutic adenoviral vector synergistically suppressed the growth of non-small cell lung cancer. J Cancer Res Clin Oncol. 2020,146(4):861-874. doi: 10.1007/s00432-020-03157-2
    Read this paper
  • Celik-Ozenci C, Kuscu N, Gungor-Ordueri NE, et al. Inhibition of poly(ADP-ribose) polymerase may have preventive potential for varicocoele-associated testicular damage in rats. Andrology. 2017,5(2):362-369. doi: 10.1111/andr.12305
    Read this paper
  • Uchiumi F, Shoji K, Sasaki Y, et al. Characterization of the 5'-flanking region of the human TP53 gene and its response to the natural compound, Resveratrol. J Biochem. 2016,159(4):437-47. doi: 10.1093/jb/mvv126
    Read this paper
  • Carry PY, Banssillon V. [Intra-abdominal pressure]. Ann Fr Anesth Reanim. 1994,13(3):381-99.
    Read this paper

Datasheet

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Figure 1. Western blot analysis of TP53 using anti-TP53 antibody (A00001-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53 antigen affinity purified polyclonal antibody (Catalog # A00001-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53 at approximately 53 kDa. The expected band size for TP53 is at 44 kDa.
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