More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/p53 (TP53) (16-25) Mouse Monoclonal Antibody [Clone ID: X77]
Figure 1. Western blot analysis using p53 antibody at 10 ug/ml on HCT116 cell lysate (Lane 1), HCT116 cell lysate activated with adriamycin (Lane 2), p21-/- cell lysate (Lane 3), P21-/- cell lysate activated with ADR (Lane 4) and p53-/- activated with ADR (Lane 5). ADR activates p53 in cells. Also shown is a graphic representation of the epitope location.
Figure 1. Western blot analysis using p53 antibody at 10 ug/ml on HCT116 cell lysate (Lane 1), HCT116 cell lysate activated with adriamycin (Lane 2), p21-/- cell lysate (Lane 3), P21-/- cell lysate activated with ADR (Lane 4) and p53-/- activated with ADR (Lane 5). ADR activates p53 in cells. Also shown is a graphic representation of the epitope location.
Figure 1. Western blot analysis using p53 antibody at 10 ug/ml on HCT116 cell lysate (Lane 1), HCT116 cell lysate activated with adriamycin (Lane 2), p21-/- cell lysate (Lane 3), P21-/- cell lysate activated with ADR (Lane 4) and p53-/- activated with ADR (Lane 5). ADR activates p53 in cells. Also shown is a graphic representation of the epitope location.

p53 (TP53) (16-25) Mouse Monoclonal Antibody [Clone ID: X77]

AM05279PU-N
OriGene
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Xenopus
TargetTP53
Price on request
100 ug
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    p53 (TP53) (16-25) Mouse Monoclonal Antibody [Clone ID: X77]
  • Delivery Days Customer
    14
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    X77
  • Gene ID7157
  • Target name
    TP53
  • Target description
    tumor protein p53
  • Target synonyms
    BCC7, BMFS5, LFS1, P53, TRP53, cellular tumor antigen p53, antigen NY-CO-13, mutant tumor protein 53, phosphoprotein p53, transformation-related protein 53, tumor protein 53, tumor supressor p53
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP04637
  • Protein Name
    Cellular tumor antigen p53
  • Scientific Description
    p53 (TP53) (16-25) mouse monoclonal antibody, clone X77, Purified
  • Reactivity
    Human, Mouse, Xenopus
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Absolute Antibody
Anti-p53 [PAb421]Ab00142-2.0
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
ReactivityHuman, Monkey, Mouse, Rabbit, Rat
TargetTP53
  • SizePrice
Product group Antibodies
Biosynth
P53 antibody10R-10381
  • SizePrice
Anti-p53 Antibody
Product group Antibodies
Antibodies.com
Anti-p53 AntibodyA101571
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Product group Antibodies
RayBiotech
Anti-P53 Antibody130-10102
ApplicationsELISA
  • SizePrice
Immunohistochemistry analysis in human skin and skeletal muscle tissues using AMAb90956 antibody. Corresponding p53 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-p53 AntibodyAMAB90956
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTP53
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
TP53 / p53 Antibody (clone 2C3-H6-H11, Preservative Free)LS-C743719
ApplicationsELISA
ReactivityHuman
TargetTP53
  • SizePrice
Figure 1. Western blot analysis of TP53 using anti-TP53 antibody (A00001-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53 antigen affinity purified polyclonal antibody (Catalog # A00001-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53 at approximately 53 kDa. The expected band size for TP53 is at 44 kDa.
Product group Antibodies
Boster Bio
Anti-TP53 Antibody Picoband(r)A00001-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetTP53
  • SizePrice
Formalin-fixed and paraffin embedded human ovarian carcinoma labeled with Rabbit Anti-P53 protein(wt-p53) Polyclonal Antibody (bs-0033R), Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining
Product group Antibodies
References
Bioss
P53 Polyclonal AntibodyBS-0033R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetTP53
  • SizePrice
IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
Product group Antibodies
Cusabio
TP53 Monoclonal AntibodyCSB-MA000208
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetTP53
  • SizePrice
Product group Antibodies
Biomatik
Tp53 Polyclonal AntibodyCAC07006
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetTP53
  • SizePrice