Detection of activated p53 Recombinant C-terminal fragment of p53 was incubated with Casein Kinase II in the absence (1) or presence (2) of ATP. Proteins were separarted by SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with Monoclonal p53 antibody (clone 9F4) (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).