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WB analysis of 293 cell lysate using GTX31859 p57 Kip2 antibody. Working concentration : (A) 1 and (B) 2 microg/ml
WB analysis of 293 cell lysate using GTX31859 p57 Kip2 antibody. Working concentration : (A) 1 and (B) 2 microg/ml
WB analysis of 293 cell lysate using GTX31859 p57 Kip2 antibody. Working concentration : (A) 1 and (B) 2 microg/ml

p57 Kip2 antibody

GTX31859
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetCDKN1C
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Overview

  • Supplier
    GeneTex
  • Product Name
    p57 Kip2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 2 microg/mL. ICC/IF: 20 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1028
  • Target name
    CDKN1C
  • Target description
    cyclin dependent kinase inhibitor 1C
  • Target synonyms
    BWCR; BWS; cyclin-dependent kinase inhibitor 1C; cyclin-dependent kinase inhibitor 1C (p57, Kip2); cyclin-dependent kinase inhibitor p57; KIP2; p57; p57Kip2; WBS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49918
  • Protein Name
    Cyclin-dependent kinase inhibitor 1C
  • Scientific Description
    This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Effect of Butyrate on Collagen Expression, Cell Viability, Cell Cycle Progression and Related Proteins Expression of MG-63 Osteoblastic Cells. Chang MC et al., 2016, PLoS One
    Read more

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Figure 1. Western blot analysis of p57 Kip2/CDKN1C using anti-p57 Kip2/CDKN1C antibody (A01244-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p57 Kip2/CDKN1C antigen affinity purified polyclonal antibody (Catalog # A01244-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p57 Kip2/CDKN1C at approximately 57KD. The expected band size for p57 Kip2/CDKN1C is at 57KD.
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