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ProductsBack/Mouse anti Human Heterogeneous Nuclear Ribonucleoprotein K

Mouse anti Human Heterogeneous Nuclear Ribonucleoprotein K

ORB109167
Biorbyt
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetHNRNPK
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Mouse anti Human Heterogeneous Nuclear Ribonucleoprotein K
  • Delivery Days Customer
    16
  • Application Supplier Note
    Antibody can be used for Western blotting (1-2 microg/ml) and immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-5 microg/ml). Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    F45 P9 C7
  • Conjugate
    Unconjugated
  • Gene ID3190
  • Target name
    HNRNPK
  • Target description
    heterogeneous nuclear ribonucleoprotein K
  • Target synonyms
    AUKS, CSBP, HNRPK, TUNP, heterogeneous nuclear ribonucleoprotein K, dC-stretch binding protein, transformation upregulated nuclear protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP61978
  • Protein Name
    Heterogeneous nuclear ribonucleoprotein K
  • Scientific Description
    Mouse monoclonal to P61978
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of hnRNP K/HNRNPK using anti-hnRNP K/HNRNPK antibody (A01793-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-hnRNP K/HNRNPK antigen affinity purified polyclonal antibody (Catalog # A01793-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for hnRNP K/HNRNPK at approximately 60 kDa. The expected band size for hnRNP K/HNRNPK is at 55 kDa.
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