WB (peptide competition) analysis of Jurkat cells using GTX25231 p70 S6K (phospho Thr229) antibody prior incubated with the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphothreonine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.
WB (peptide competition) analysis of Jurkat cells using GTX25231 p70 S6K (phospho Thr229) antibody prior incubated with the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphothreonine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.
p70 S6K (phospho Thr229) antibody
GTX25231
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetRPS6KB1
Overview
- SupplierGeneTex
- Product Namep70 S6K (phospho Thr229) antibody - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteICC/IF: 1:250. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID6198
- Target nameRPS6KB1
- Target descriptionribosomal protein S6 kinase B1
- Target synonymsp70 S6KA; p70(S6K)-alpha; p70-alpha; p70-S6K; PS6K; ribosomal protein S6 kinase beta-1; ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kDa, polypeptide 1; S6K; S6K1; S6K-beta-1; serine/threonine kinase 14 alpha; serine/threonine-protein kinase 14A; STK14A
- HostRabbit
- IsotypeIgG
- Protein IDP23443
- Protein NameRibosomal protein S6 kinase beta-1
- Scientific DescriptionThis gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]
- ReactivityHuman, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- The PI3K and MAPK/p38 pathways control stress granule assembly in a hierarchical manner. Heberle AM et al., 2019 Apr, Life Sci AllianceRead more
- mTOR is involved in stroke-induced seizures and the anti-seizure effect of mild hypothermia. Yang GS et al., 2018 Apr, Mol Med RepRead more
- A systems study reveals concurrent activation of AMPK and mTOR by amino acids. Dalle Pezze P et al., 2016 Nov 21, Nat CommunRead more