FACS (Intracellular staining) analysis of HeLa cells using GTX46123 p73 antibody [5B429]. Blue : Primary antibody Orange : isotype control Dilution : 1 microg/mL Fixation : 4% PFA Permibilization : 0.1% saponin
![ICC/IF analysis of HeLa cells using GTX46123 p73 antibody [5B429]. Red : primary antibody Blue : DAPI Dilution : 3 ug/mL](https://www.genetex.com/upload/website/prouct_img/normal/GTX46123/GTX46123_1605_ICC-IF_w_23060820_845.webp "ICC/IF analysis of HeLa cells using GTX46123 p73 antibody [5B429]. Red : primary antibody Blue : DAPI Dilution : 3 ug/mL")
![WB analysis of (A)HeLa overexpressing p73 (B) HeLa mock cell lysate using GTX46123 p73 antibody [5B429]. Dilution : 2 microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX46123/GTX46123_1641_WB_w_23060820_430.webp "WB analysis of (A)HeLa overexpressing p73 (B) HeLa mock cell lysate using GTX46123 p73 antibody [5B429]. Dilution : 2 microg/ml")
![WB analysis of HeLa cell lysate using GTX46123 p73 antibody [5B429].](https://www.genetex.com/upload/website/prouct_img/normal/GTX46123/GTX46123_1642_WB_w_23060820_625.webp "WB analysis of HeLa cell lysate using GTX46123 p73 antibody [5B429].")
![IHC-P analysis of human breast carcinoma tissue using GTX46123 p73 antibody [5B429]. Dilution : 5 microg/ml Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0](https://www.genetex.com/upload/website/prouct_img/normal/GTX46123/GTX46123_604_IHC-P_w_23060820_111.webp "IHC-P analysis of human breast carcinoma tissue using GTX46123 p73 antibody [5B429]. Dilution : 5 microg/ml Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0")
FACS (Intracellular staining) analysis of HeLa cells using GTX46123 p73 antibody [5B429]. Blue : Primary antibody Orange : isotype control Dilution : 1 microg/mL Fixation : 4% PFA Permibilization : 0.1% saponin
p73 antibody [5B429]
GTX46123
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Mass Spectrometry, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTP73
Overview
- SupplierGeneTex
- Product Namep73 antibody [5B429] - Overexpression Validated
- Delivery Days Customer9
- Antibody Specificityp73 antibody (clone 5B429) recognizes all of the TAp73 isoforms i.e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 antibody (clone 5B429) reacted with
- Application Supplier NoteWB: 2 microg/ml. ICC/IF: 1:20 - 1:1000. IHC-P: 5 - 15 microg/ml. IHC-Fr: 5 - 15 microg/ml. FACS: 1 microg/ml. IP: 1:20 - 1:1000. ChIP assay: 1:20 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Mass Spectrometry, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5B429
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7161
- Target nameTP73
- Target descriptiontumor protein p73
- Target synonymsCILD47; p53-like transcription factor; p53-related protein; P73; tumor protein p73
- HostMouse
- IsotypeIgG1
- Protein IDO15350
- Protein NameTumor protein p73
- Scientific DescriptionThis gene encodes tumor protein p73, which is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53, p63, and p73 and have high sequence similarity to one another, which allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways involving direct or indirect protein interactions, resulting in regulation of the same target gene promoters or regulation of each others promoters. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors. The p73 gene expresses at least 35 mRNA variants due to the use of alternate promoters, alternate translation initiation sites, and multiple splice variations. Theoretically this can account for 29 different p73 isoforms; however, the biological validity and the full-length nature of most variants have not been determined. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203