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IHC-Fr analysis of rat brain tissue using GTX37576 PACAP antibody.
IHC-Fr analysis of rat brain tissue using GTX37576 PACAP antibody.
IHC-Fr analysis of rat brain tissue using GTX37576 PACAP antibody.

PACAP antibody

GTX37576
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetADCYAP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    PACAP antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:400-800. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID116
  • Target name
    ADCYAP1
  • Target description
    adenylate cyclase activating polypeptide 1
  • Target synonyms
    adenylate cyclase activating polypeptide 1 (pituitary); PACAP; pituitary adenylate cyclase-activating polypeptide; prepro-PACAP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18509
  • Protein Name
    Pituitary adenylate cyclase-activating polypeptide
  • Scientific Description
    This gene encodes a secreted proprotein that is further processed into multiple mature peptides. These peptides stimulate adenylate cyclase and increase cyclic adenosine monophosphate (cAMP) levels, resulting in the transcriptional activation of target genes. The products of this gene are key mediators of neuroendocrine stress responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Early Alterations of PACAP and VIP Expression in the Female Rat Brain Following Spinal Cord Injury.
    Read more
  • PACAP and VIP Mitigate Rotenone-Induced Inflammation in BV-2 Microglial Cells.
    Read more
  • Ameliorative effects of PACAP against cartilage degeneration. Morphological, immunohistochemical and biochemical evidence from in vivo and in vitro models of rat osteoarthritis. Giunta S et al., 2015 Mar 13, Int J Mol Sci
    Read more

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Figure 1. Western blot analysis of PACAP-38/ADCYAP1 using anti-PACAP-38/ADCYAP1 antibody (A01711-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PACAP-38/ADCYAP1 antigen affinity purified polyclonal antibody (Catalog # A01711-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PACAP-38/ADCYAP1 at approximately 19 kDa. The expected band size for PACAP-38/ADCYAP1 is at 19 kDa.
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