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PACAP Antibody

LS-C813919
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetADCYAP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PACAP Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:20000), IHC Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID116
  • Target name
    ADCYAP1
  • Target description
    adenylate cyclase activating polypeptide 1
  • Target synonyms
    adenylate cyclase activating polypeptide 1 (pituitary); PACAP; pituitary adenylate cyclase-activating polypeptide; prepro-PACAP
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PACAP-38/ADCYAP1 using anti-PACAP-38/ADCYAP1 antibody (A01711-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PACAP-38/ADCYAP1 antigen affinity purified polyclonal antibody (Catalog # A01711-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PACAP-38/ADCYAP1 at approximately 19 kDa. The expected band size for PACAP-38/ADCYAP1 is at 19 kDa.
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