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ProductsBack/PAICS Mouse Monoclonal Antibody [Clone ID: OTI1B4]
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells (WT) and PAICS-Knockdown HeLa cells (KD) were separated by SDS-PAGE and immunoblotted with anti-PAICS monoclonal antibody TA501470 (1:2500). Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells (WT) and PAICS-Knockdown HeLa cells (KD) were separated by SDS-PAGE and immunoblotted with anti-PAICS monoclonal antibody TA501470 (1:2500). Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells (WT) and PAICS-Knockdown HeLa cells (KD) were separated by SDS-PAGE and immunoblotted with anti-PAICS monoclonal antibody TA501470 (1:2500). Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.

PAICS Mouse Monoclonal Antibody [Clone ID: OTI1B4]

CF501470
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPAICS
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    PAICS Mouse Monoclonal Antibody [Clone ID: OTI1B4]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1B4
  • Gene ID10606
  • Target name
    PAICS
  • Target description
    phosphoribosylaminoimidazole carboxylase and phosphoribosylaminoimidazolesuccinocarboxamide synthase
  • Target synonyms
    ADE2, ADE2H1, AIRC, PAICSD, PAIS, bifunctional phosphoribosylaminoimidazole carboxylase/phosphoribosylaminoimidazole succinocarboxamide synthetase, AIR carboxylase, SAICAR synthetase, multifunctional protein ADE2, multifunctional protein ADE2H1, phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase, phosphoribosylaminoimidazole carboxylase; phosphoribosylaminoimidazolesuccinocarboxamide synthase
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP22234
  • Protein Name
    Bifunctional phosphoribosylaminoimidazole carboxylase/phosphoribosylaminoimidazole succinocarboxamide synthetase
  • Scientific Description
    Carrier-free (BSA/glycerol-free) PAICS mouse monoclonal antibody, clone OTI1B4 (formerly 1B4)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PAICS using anti-PAICS antibody (A07031-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAICS antigen affinity purified polyclonal antibody (Catalog # A07031-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAICS at approximately 47 kDa. The expected band size for PAICS is at 47 kDa.
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