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Western Blot analysis of 293 cells using PAKalpha Polyclonal Antibody
Western Blot analysis of 293 cells using PAKalpha Polyclonal Antibody
Western Blot analysis of 293 cells using PAKalpha Polyclonal Antibody

PAK1 Antibody

CSB-PA003703
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPAK1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PAK1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5058
  • Target name
    PAK1
  • Target description
    p21 (RAC1) activated kinase 1
  • Target synonyms
    alpha-PAK; IDDMSSD; p21 protein (Cdc42/Rac)-activated kinase 1; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p65-PAK; PAKalpha; serine/threonine-protein kinase PAK 1; STE20 homolog, yeast
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13153
  • Protein Name
    Serine/threonine-protein kinase PAK 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PAK1 using anti-PAK1 antibody (A00454-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK1 antigen affinity purified polyclonal antibody (Catalog # A00454-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAK1 at approximately 66KD. The expected band size for PAK1 is at 66KD.
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