Bio-Connect

PAK1 antibody

GTX103797
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPAK1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    PAK1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.48 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5058
  • Target name
    PAK1
  • Target description
    p21 (RAC1) activated kinase 1
  • Target synonyms
    alpha-PAK; IDDMSSD; p21 protein (Cdc42/Rac)-activated kinase 1; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p65-PAK; PAKalpha; serine/threonine-protein kinase PAK 1; STE20 homolog, yeast
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13153
  • Protein Name
    Serine/threonine-protein kinase PAK 1
  • Scientific Description
    PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • p21-activated kinase 1 activity is required for histone H3 Ser10 phosphorylation and chromatin condensation in mouse oocyte meiosis. Zhang N et al., 2017 Jul, Reprod Fertil Dev
    Read more

Related products

Product group Antibodies
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetPAK1
  • SizePrice
Dot blot analysis of phosphorylated and non-phosphorylated forms of the immunizing peptide using GTX22477 PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody. Dilution : 1:1000
Product group Antibodies
GeneTex
ApplicationsDot Blot, Western Blot, ELISA
  • SizePrice
Human PAK1 detected by Western blot in transfected HEK293 cells (left lane)
Product group Antibodies
GeneTex
ApplicationsImmunoPrecipitation, Western Blot
TargetPAK1
  • SizePrice
WB analysis of whole cell extracts (30 microg lysate) of SH-SY5Y (Lane 1) treated with 100 uM Pervanadate for 1 hour (Lane 2), HeLa (Lane 3) treated with 100 uM Pervanadate for 1 hour (Lane 4), K562 (Lane 5) treated with 100 uM Pervanadate for 1 hour (Lane 6) using GTX25247 PAK2 (phospho Ser141) antibody. Dilution : 1:500
Product group Antibodies
References
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
TargetPAK1
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetPAK1
  • SizePrice
Western Blot analysis of 293 cells using PAKalpha Polyclonal Antibody
Product group Antibodies
Cusabio
PAK1 AntibodyCSB-PA003703
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPAK1
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetPAK1
  • SizePrice
Product group Antibodies
Everest Biotech
ApplicationsWestern Blot, ELISA
TargetPAK1
  • SizePrice
Figure 1. Western blot analysis of PAK1 using anti-PAK1 antibody (A00454-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK1 antigen affinity purified polyclonal antibody (Catalog # A00454-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAK1 at approximately 66KD. The expected band size for PAK1 is at 66KD.
Product group Antibodies
Boster Bio
Anti-PAK1 Antibody Picoband(r)A00454-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetPAK1
  • SizePrice
ELISA detection of PAK1 using GTX111076 for capture and GTX89274 for detection at a concentration of 3.2 microg/mL. The HRP-conjugated anti-goat IgG antibody (GTX26741) was diluted at 1:20,000-1:200,000 and used to detect the primary antibody.
Product group Antibodies
GeneTex
ApplicationsELISA
TargetPAK1
  • SizePrice