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Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Palladin antibody (GTX135128) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Palladin antibody (GTX135128) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Palladin antibody (GTX135128) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Palladin antibody

GTX135128
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPALLD
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Overview

  • Supplier
    GeneTex
  • Product Name
    Palladin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.56 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23022
  • Target name
    PALLD
  • Target description
    palladin, cytoskeletal associated protein
  • Target synonyms
    CGI-151, CGI151, MYN, PNCA1, SIH002, palladin, myoneurin, sarcoma antigen NY-SAR-77
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WX93
  • Protein Name
    Palladin
  • Scientific Description
    This gene encodes a cytoskeletal protein that is required for organizing the actin cytoskeleton. The protein is a component of actin-containing microfilaments, and it is involved in the control of cell shape, adhesion, and contraction. Polymorphisms in this gene are associated with a susceptibility to pancreatic cancer type 1, and also with a risk for myocardial infarction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Palladin/PALLD using anti-Palladin/PALLD antibody (A04005-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Palladin/PALLD antigen affinity purified polyclonal antibody (Catalog # A04005-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Palladin/PALLD at approximately 95 kDa. The expected band size for Palladin/PALLD is at 151 kDa.
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