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ProductsBack/PALLD / Palladin Antibody

PALLD / Palladin Antibody

LS-C747473
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPALLD
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PALLD / Palladin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 42kDa/58kDa/73kDa/85-150kDa, while the observed MW by Western blot was 150kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 42kDa/58kDa/73kDa/85-150kDa, while the observed MW by Western blot was 150kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23022
  • Target name
    PALLD
  • Target description
    palladin, cytoskeletal associated protein
  • Target synonyms
    CGI151; CGI-151; MYN; myoneurin; palladin; PNCA1; sarcoma antigen NY-SAR-77; SIH002
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Palladin/PALLD using anti-Palladin/PALLD antibody (A04005-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Palladin/PALLD antigen affinity purified polyclonal antibody (Catalog # A04005-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Palladin/PALLD at approximately 95 kDa. The expected band size for Palladin/PALLD is at 151 kDa.
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Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & actin filaments.
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