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ProductsBack/PAN2 / USP52 Antibody

PAN2 / USP52 Antibody

LS-C750343
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPAN2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PAN2 / USP52 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 135kDa, while the observed MW by Western blot was 135kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:200 - 1:2000) The predicted MW is 135kDa, while the observed MW by Western blot was 135kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9924
  • Target name
    PAN2
  • Target description
    poly(A) specific ribonuclease subunit PAN2
  • Target synonyms
    USP52, PAN2-PAN3 deadenylation complex catalytic subunit PAN2, PAB-dependent poly(A)-specific ribonuclease subunit 2, PAB-dependent poly(A)-specific ribonuclease subunit PAN2, PAB1P-dependent poly(A)-nuclease, PAB1P-dependent poly(A)-specific ribonuclease, PABP-dependent poly(A) nuclease 2, PAN deadenylation complex catalytic subunit 2, PAN deadenylation complex subunit 2, PAN2 poly(A) specific ribonuclease subunit homolog, inactive ubiquitin carboxyl-terminal hydrolase 52, poly(A)-nuclease deadenylation complex subunit 2, ubiquitin specific peptidase 52, ubiquitin specific protease 52
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PAN2 using anti-PAN2 antibody (A03834-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hel whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAN2 antigen affinity purified polyclonal antibody (Catalog # A03834-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAN2 at approximately 135 kDa. The expected band size for PAN2 is at 135 kDa.
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