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ProductsBack/Parathyroid Hormone Receptor 1 (PTH1R) Mouse Monoclonal Antibody [Clone ID: 4D2]
Western blot analysis using PTH1R antibody Cat.-No AM06494SU-Nagainst PTH1R (AA: 27-188)-hIgGFc transfected HEK293 cell lysate.
Western blot analysis using PTH1R antibody Cat.-No AM06494SU-Nagainst PTH1R (AA: 27-188)-hIgGFc transfected HEK293 cell lysate.
Western blot analysis using PTH1R antibody Cat.-No AM06494SU-Nagainst PTH1R (AA: 27-188)-hIgGFc transfected HEK293 cell lysate.

Parathyroid Hormone Receptor 1 (PTH1R) Mouse Monoclonal Antibody [Clone ID: 4D2]

AM06494SU-N
OriGene
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPTH1R
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Parathyroid Hormone Receptor 1 (PTH1R) Mouse Monoclonal Antibody [Clone ID: 4D2]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4D2
  • Gene ID5745
  • Target name
    PTH1R
  • Target description
    parathyroid hormone 1 receptor
  • Target synonyms
    EKNS, PFE, PTHR, PTHR1, parathyroid hormone/parathyroid hormone-related peptide receptor, PTH/PTHr receptor, PTH/PTHrP type I receptor, PTH1 receptor, parathyroid hormone receptor 1, parathyroid hormone/parathyroid hormone-related protein receptor, seven transmembrane helix receptor
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ03431
  • Protein Name
    Parathyroid hormone/parathyroid hormone-related peptide receptor
  • Scientific Description
    Parathyroid Hormone Receptor 1 (PTH1R) mouse monoclonal antibody, clone 4D2, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Parathyroid Hormone Receptor 1/PTH1R using anti-Parathyroid Hormone Receptor 1/PTH1R antibody (A01588). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HUH-7 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Parathyroid Hormone Receptor 1/PTH1R antigen affinity purified polyclonal antibody (Catalog # A01588) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Parathyroid Hormone Receptor 1/PTH1R at approximately 66 kDa. The expected band size for Parathyroid Hormone Receptor 1/PTH1R is at 66 kDa.
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