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ProductsBack/PARP antibody [GT982]
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT982] (GTX632388) diluted at 1:1000.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT982] (GTX632388) diluted at 1:1000.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT982] (GTX632388) diluted at 1:1000.

PARP antibody [GT982]

GTX632388
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPARP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    PARP antibody [GT982]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT982
  • Concentration
    1.65 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID142
  • Target name
    PARP1
  • Target description
    poly(ADP-ribose) polymerase 1
  • Target synonyms
    ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1, poly [ADP-ribose] polymerase 1, ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase), ADP-ribosyltransferase NAD(+), ADP-ribosyltransferase diphtheria toxin-like 1, DNA ADP-ribosyltransferase PARP1, NAD(+) ADP-ribosyltransferase 1, poly (ADP-ribose) polymerase family, member 1, poly(ADP-ribose) synthetase, poly(ADP-ribosyl)transferase, poly[ADP-ribose] synthase 1, protein poly-ADP-ribosyltransferase PARP1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP09874
  • Protein Name
    Poly [ADP-ribose] polymerase 1
  • Scientific Description
    This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Sikorski K, Mehta A, Inngjerdingen M, et al. A high-throughput pipeline for validation of antibodies. Nat Methods. 2018,15(11):909-912. doi: 10.1038/s41592-018-0179-8
    Read this paper

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Figure 1. Western blot analysis of PARP1 using anti-PARP1 antibody (A00122-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP1 antigen affinity purified polyclonal antibody (Catalog # A00122-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP1 at approximately 113 kDa. The expected band size for PARP1 is at 113 kDa.
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