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Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

PARP1 Polyclonal Antibody

BS-2138R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetPARP1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    PARP1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID142
  • Target name
    PARP1
  • Target description
    poly(ADP-ribose) polymerase 1
  • Target synonyms
    ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT; ADPRT 1; ADPRT1; ARTD1; DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1; pADPRT-1; PARP; PARP-1; poly (ADP-ribose) polymerase family, member 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; poly[ADP-ribose] synthase 1; PPOL; protein poly-ADP-ribosyltransferase PARP1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09874
  • Protein Name
    Poly [ADP-ribose] polymerase 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Jiawei Foshou San Induces Apoptosis in Ectopic Endometrium Based on Systems Pharmacology, Molecular Docking, and Experimental Evidence. Wei J et al., 2019, Evid Based Complement Alternat Med
    Read more
  • FHL1 regulates myoblast differentiation and autophagy through its interaction with LC3. Han S et al., 2020 May, J Cell Physiol
    Read more
  • Characterization and Inception of a Triterpenoid Astrakurkurol, as a Cytotoxic Molecule on Human Hepatocellular Carcinoma Cells, Hep3B. Nandi S et al., 2019 Jul 10, J Agric Food Chem
    Read more
  • Vitamin E improves testicular damage in streptozocin-induced diabetic rats, via increasing vascular endothelial growth factor and poly(ADP-ribose) polymerase-1. Omar SS et al., 2018 Apr, Andrologia
    Read more
  • Platelet-rich plasma protects rat chondrocytes from interleukin-1beta-induced apoptosis. Yang J et al., 2016 Nov, Mol Med Rep
    Read more
  • Crucial Role of Lateral Size for Graphene Oxide in Activating Macrophages and Stimulating Pro-inflammatory Responses in Cells and Animals. Ma J et al., 2015 Oct 27, ACS Nano
    Read more
  • Echinacoside Induces Apoptosis in Human SW480 Colorectal Cancer Cells by Induction of Oxidative DNA Damages. Dong L et al., 2015 Jun 29, Int J Mol Sci
    Read more
  • Azithromycin synergistically enhances anti-proliferative activity of vincristine in cervical and gastric cancer cells. Zhou X et al., 2012 Dec 4, Cancers (Basel)
    Read more

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Figure 1. Western blot analysis of PARP1 using anti-PARP1 antibody (A00122-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP1 antigen affinity purified polyclonal antibody (Catalog # A00122-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP1 at approximately 113 kDa. The expected band size for PARP1 is at 113 kDa.
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