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Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

PARP1 Polyclonal Antibody

BS-2138R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPARP1
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Overview

  • Supplier
    Bioss
  • Product Name
    PARP1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID142
  • Target name
    PARP1
  • Target description
    poly(ADP-ribose) polymerase 1
  • Target synonyms
    ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1, poly [ADP-ribose] polymerase 1, ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase), ADP-ribosyltransferase NAD(+), ADP-ribosyltransferase diphtheria toxin-like 1, DNA ADP-ribosyltransferase PARP1, NAD(+) ADP-ribosyltransferase 1, poly (ADP-ribose) polymerase family, member 1, poly(ADP-ribose) synthetase, poly(ADP-ribosyl)transferase, poly[ADP-ribose] synthase 1, protein poly-ADP-ribosyltransferase PARP1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09874
  • Protein Name
    Poly [ADP-ribose] polymerase 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Wei J, Zhao B, Zhang C, et al. Jiawei Foshou San Induces Apoptosis in Ectopic Endometrium Based on Systems Pharmacology, Molecular Docking, and Experimental Evidence. Evid Based Complement Alternat Med. 2019,2019:2360367. doi: 10.1155/2019/2360367
    Read this paper
  • Han S, Cui C, He H, et al. FHL1 regulates myoblast differentiation and autophagy through its interaction with LC3. J Cell Physiol. 2020,235(5):4667-4678. doi: 10.1002/jcp.29345
    Read this paper
  • Nandi S, Chandra S, Sikder R, et al. Characterization and Inception of a Triterpenoid Astrakurkurol, as a Cytotoxic Molecule on Human Hepatocellular Carcinoma Cells, Hep3B. J Agric Food Chem. 2019,67(27):7660-7673. doi: 10.1021/acs.jafc.9b01203
    Read this paper
  • Omar SS, Aly RG, Badae NM. Vitamin E improves testicular damage in streptozocin-induced diabetic rats, via increasing vascular endothelial growth factor and poly(ADP-ribose) polymerase-1. Andrologia. 2018,50(3). doi: 10.1111/and.12925
    Read this paper
  • Yang J, Lu Y, Guo A. Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis. Mol Med Rep. 2016,14(5):4075-4082. doi: 10.3892/mmr.2016.5767
    Read this paper
  • Ma J, Liu R, Wang X, et al. Crucial Role of Lateral Size for Graphene Oxide in Activating Macrophages and Stimulating Pro-inflammatory Responses in Cells and Animals. ACS Nano. 2015,9(10):10498-515. doi: 10.1021/acsnano.5b04751
    Read this paper
  • Dong L, Yu D, Wu N, et al. Echinacoside Induces Apoptosis in Human SW480 Colorectal Cancer Cells by Induction of Oxidative DNA Damages. Int J Mol Sci. 2015,16(7):14655-68. doi: 10.3390/ijms160714655
    Read this paper
  • Zhou X, Zhang Y, Li Y, et al. Azithromycin synergistically enhances anti-proliferative activity of vincristine in cervical and gastric cancer cells. Cancers (Basel). 2012,4(4):1318-32. doi: 10.3390/cancers4041318
    Read this paper

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