More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/PARP1 Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

PARP1 Polyclonal Antibody

BS-2138R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPARP1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    PARP1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID142
  • Target name
    PARP1
  • Target description
    poly(ADP-ribose) polymerase 1
  • Target synonyms
    ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1, poly [ADP-ribose] polymerase 1, ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase), ADP-ribosyltransferase NAD(+), ADP-ribosyltransferase diphtheria toxin-like 1, DNA ADP-ribosyltransferase PARP1, NAD(+) ADP-ribosyltransferase 1, poly (ADP-ribose) polymerase family, member 1, poly(ADP-ribose) synthetase, poly(ADP-ribosyl)transferase, poly[ADP-ribose] synthase 1, protein poly-ADP-ribosyltransferase PARP1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09874
  • Protein Name
    Poly [ADP-ribose] polymerase 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • Jiawei Foshou San Induces Apoptosis in Ectopic Endometrium Based on Systems Pharmacology, Molecular Docking, and Experimental Evidence. Wei J et al., 2019, Evid Based Complement Alternat Med
    Read this paper
  • FHL1 regulates myoblast differentiation and autophagy through its interaction with LC3. Han S et al., 2020 May, J Cell Physiol
    Read this paper
  • Characterization and Inception of a Triterpenoid Astrakurkurol, as a Cytotoxic Molecule on Human Hepatocellular Carcinoma Cells, Hep3B. Nandi S et al., 2019 Jul 10, J Agric Food Chem
    Read this paper
  • Vitamin E improves testicular damage in streptozocin-induced diabetic rats, via increasing vascular endothelial growth factor and poly(ADP-ribose) polymerase-1. Omar SS et al., 2018 Apr, Andrologia
    Read this paper
  • Platelet-rich plasma protects rat chondrocytes from interleukin-1beta-induced apoptosis. Yang J et al., 2016 Nov, Mol Med Rep
    Read this paper
  • Crucial Role of Lateral Size for Graphene Oxide in Activating Macrophages and Stimulating Pro-inflammatory Responses in Cells and Animals. Ma J et al., 2015 Oct 27, ACS Nano
    Read this paper
  • Echinacoside Induces Apoptosis in Human SW480 Colorectal Cancer Cells by Induction of Oxidative DNA Damages. Dong L et al., 2015 Jun 29, Int J Mol Sci
    Read this paper
  • Azithromycin synergistically enhances anti-proliferative activity of vincristine in cervical and gastric cancer cells. Zhou X et al., 2012 Dec 4, Cancers (Basel)
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
PARP1 AntibodyCAB0010
ReactivityHuman
TargetPARP1
  • SizePrice
Figure 1. Western blot analysis of PARP1 using anti-PARP1 antibody (A00122-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP1 antigen affinity purified polyclonal antibody (Catalog # A00122-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP1 at approximately 113 kDa. The expected band size for PARP1 is at 113 kDa.
Product group Antibodies
Boster Bio
Anti-PARP1 Antibody Picoband(r)A00122-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetPARP1
  • SizePrice
Anti-PARP1 Antibody
Product group Antibodies
Antibodies.com
Anti-PARP1 AntibodyA11205
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
RayBiotech
Anti-PARP1 Antibody144-00942
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPARP1
  • SizePrice
Immunohistochemical staining of human tonsil shows strong nuclear positivity in lymphoid cells.
Product group Antibodies
Atlas Antibodies
Anti-PARP1 AntibodyAMAB90959
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetPARP1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PARP1 Antibody (clone 4A3)LS-C765617
ApplicationsWestern Blot
ReactivityHuman
TargetPARP1
  • SizePrice
Product group Antibodies
MedChem Express
PARP1 Antibody (YA246, Rabbit)HY-P80263
TargetPARP1
  • SizePrice
Western blot analysis of 1) Hela, 2) 293T, 3) Jurkat, diluted at 1:2000.
Product group Antibodies
Cusabio
PARP1 Monoclonal AntibodyCSB-MA000290
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetPARP1
  • SizePrice
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:2000.
Product group Antibodies
GeneTex
PARP antibodyGTX100573
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetPARP1
  • SizePrice
Product group Antibodies
Applied Biological Materials
Anti-PARP1Y058156
ApplicationsWestern Blot, ELISA
ReactivityHuman
  • SizePrice