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Western blot analysis of extracts of various cell lines using PARP10 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using PARP10 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using PARP10 Polyclonal Antibody at dilution of 1:3000.

PARP10 Polyclonal Antibody

E-AB-64335
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    PARP10 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    Poly(ADP-ribose) polymerases (PARPs), such as PARP10, regulate gene transcription by altering chromatin organization by adding ADP-ribose to histones. PARPs can also function as transcriptional cofactors.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PARP10 using anti-PARP10 antibody (A08514-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP10 antigen affinity purified polyclonal antibody (Catalog # A08514-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP10 at approximately 140 kDa. The expected band size for PARP10 is at 110 kDa.
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