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ProductsBack/Parvin alpha (PARVA) (C-term) Rabbit Polyclonal Antibody
Western blot analysis of PARVA Antibody (C-term) in mouse bladder tissue lysates (35ug/lane). PARVA (arrow) was detected using the purified Pab.
Western blot analysis of PARVA Antibody (C-term) in mouse bladder tissue lysates (35ug/lane). PARVA (arrow) was detected using the purified Pab.
Western blot analysis of PARVA Antibody (C-term) in mouse bladder tissue lysates (35ug/lane). PARVA (arrow) was detected using the purified Pab.

Parvin alpha (PARVA) (C-term) Rabbit Polyclonal Antibody

AP53170PU-N
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPARVA
400 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Parvin alpha (PARVA) (C-term) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID55742
  • Target name
    PARVA
  • Target description
    parvin alpha
  • Target synonyms
    CH-ILKBP, MXRA2, alpha-parvin, actopaxin, calponin-like integrin-linked kinase-binding protein, matrix-remodeling-associated protein 2
  • Host
    Rabbit
  • Protein IDQ9NVD7
  • Protein Name
    Alpha-parvin
  • Scientific Description
    Parvin alpha (PARVA) (C-term) rabbit polyclonal antibody, Aff - Purified
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Parvin alpha using anti-Parvin alpha antibody (PB9555). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: HELA Whole Cell Lysate at 40ug, Lane 3: SW620 Whole Cell Lysate at 40ug, Lane 4: HEPA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Parvin alpha antigen affinity purified polyclonal antibody (Catalog # PB9555) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Parvin alpha at approximately 42 kDa. The expected band size for Parvin alpha is at 42 kDa.
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