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ProductsBack/PathPlus(tm) ACHE / Acetylcholinesterase Antibody

PathPlus(tm) ACHE / Acetylcholinesterase Antibody

LS-B15531
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetACHE
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) ACHE / Acetylcholinesterase Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 58kDa/65kDa/67kDa, while the observed MW by Western blot was 75kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:100), WB (1:500 - 1:2000) The predicted MW is 58kDa/65kDa/67kDa, while the observed MW by Western blot was 75kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID43
  • Target name
    ACHE
  • Target description
    acetylcholinesterase (Yt blood group)
  • Target synonyms
    ACEE, ARACHE, N-ACHE, YT, acetylcholinesterase, Yt blood group, acetylcholinesterase (Cartwright blood group), apoptosis-related acetylcholinesterase
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACHE using anti-ACHE antibody (PB9417). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50ug, Lane 2: Mouse Liver Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: PANC Whole Cell Lysate at 40ug, Lane 5: COLO320 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACHE antigen affinity purified polyclonal antibody (Catalog # PB9417) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACHE at approximately 68 kDa. The expected band size for ACHE is at 68 kDa.
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