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ProductsBack/PathPlus(tm) ACPP / PAP Antibody (aa200-300)

PathPlus(tm) ACPP / PAP Antibody (aa200-300)

LS-B8309
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Hamster, Human, Mammals, Monkey, Mouse, Rabbit, Rat
TargetACP3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) ACPP / PAP Antibody (aa200-300)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 50kDa band observed in Human Prostate lysates, with a calculated MW of 44.6kDa according to NP_001090.2. The recommended concentration is 0.3-1 ug/ml.. IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:64000), WB (0.3 - 1 µg/ml) Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 50kDa band observed in Human Prostate lysates, with a calculated MW of 44.6kDa according to NP_001090.2. The recommended concentration is 0.3-1 ug/ml.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID55
  • Target name
    ACP3
  • Target description
    acid phosphatase 3
  • Target synonyms
    5'-NT, ACP-3, ACPP, TM-PAP, prostatic acid phosphatase, TMPase, acid phosphatase, prostate, ecto-5'-nucleotidase, prostatic acid phosphotase, protein tyrosine phosphatase ACP3, thiamine monophosphatase
  • Host
    Goat
  • Reactivity
    Bovine, Canine, Hamster, Human, Mammals, Monkey, Mouse, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PAP/ACP3 using anti-PAP/ACP3 antibody (A02082-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAP/ACP3 antigen affinity purified polyclonal antibody (Catalog # A02082-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAP/ACP3 at approximately 45-50 kDa. The expected band size for PAP/ACP3 is at 45 kDa.
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