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ProductsBack/PathPlus(tm) Alpha-1-Antichymotrypsin Antibody

PathPlus(tm) Alpha-1-Antichymotrypsin Antibody

LS-B14134
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoDiffusion, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSERPINA3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) Alpha-1-Antichymotrypsin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    This antibody is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator.
  • Applications
    Flow Cytometry, ImmunoDiffusion, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, Flo, ID, IHC, IHC-P (5 µg/ml), IP, WB This antibody is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID12
  • Target name
    SERPINA3
  • Target description
    serpin family A member 3
  • Target synonyms
    AACT, ACT, GIG24, GIG25, alpha-1-antichymotrypsin, cell growth-inhibiting gene 24/25 protein, growth-inhibiting protein 24, growth-inhibiting protein 25, serine (or cysteine) proteinase inhibitor, clade A, member 3, serpin A3, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
  • Host
    Goat
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AACT/SERPINA3 using anti-AACT/SERPINA3 antibody (A02312-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 3: human HUH-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AACT/SERPINA3 antigen affinity purified polyclonal antibody (Catalog # A02312-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AACT/SERPINA3 at approximately 50 kDa. The expected band size for AACT/SERPINA3 is at 48 kDa.
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