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ProductsBack/PathPlus(tm) BCL2L2 / Bcl-w Antibody

PathPlus(tm) BCL2L2 / Bcl-w Antibody

LS-B14858
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetBCL2L2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) BCL2L2 / Bcl-w Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 20kDa/37kDa, while the observed MW by Western blot was 20kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC (1:50 - 1:100), IHC-P (1:200), WB (1:500 - 1:2000) The predicted MW is 20kDa/37kDa, while the observed MW by Western blot was 20kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID599
  • Target name
    BCL2L2
  • Target description
    BCL2 like 2
  • Target synonyms
    BCL-W, BCL2-L-2, BCLW, PPP1R51, bcl-2-like protein 2, apoptosis regulator BCL-W, protein phosphatase 1, regulatory subunit 51
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Bcl2L2 using anti-Bcl2L2 antibody (PA1426-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U-937 whole cell lysates, Lane 4: human Hela whole cell lysates., Lane 5: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl2L2 antigen affinity purified polyclonal antibody (Catalog # PA1426-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bcl2L2 at approximately 21KD. The expected band size for Bcl2L2 is at 21KD.
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