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ProductsBack/PathPlus(tm) CBFA1 / RUNX2 Antibody (clone 4D5)

PathPlus(tm) CBFA1 / RUNX2 Antibody (clone 4D5)

LS-B4294
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetRUNX2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) CBFA1 / RUNX2 Antibody (clone 4D5)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot (Cell lysate) - positive control PC-12. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IF (10 µg/ml), IHC-P (5 µg/ml), WB Western Blot (Cell lysate) - positive control PC-12. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4D5
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID860
  • Target name
    RUNX2
  • Target description
    RUNX family transcription factor 2
  • Target synonyms
    AML3, CBF-alpha-1, CBFA1, CCD, CCD1, CLCD, OSF-2, OSF2, PEA2aA, PEBP2aA, runt-related transcription factor 2, PEA2-alpha A, PEBP2-alpha A, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit, acute myeloid leukemia 3 protein, core-binding factor, runt domain, alpha subunit 1, oncogene AML-3, osteoblast-specific transcription factor 2, polyomavirus enhancer-binding protein 2 alpha A subunit, runt related transcription factor 2
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RUNX2 using anti-RUNX2 antibody (PB9158). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human RUNX2 protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX2 antigen affinity purified polyclonal antibody (Catalog # PB9158) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX2 at approximately 50 kDa. The expected band size for RUNX2 is at 50 kDa.
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