PathPlus(tm) CD163 Antibody (clone GHI/61)

LifeSpan BioSciences

PathPlus(tm) CD163 Antibody (clone GHI/61)

23

Immunohistochemistry: LS-B3781 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3781 was determined to be 20 ug/ml. Tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.. Flo, IHC, IHC-P (20 µg/ml), WB Immunohistochemistry: LS-B3781 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3781 was determined to be 20 ug/ml. Tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

GHI/61

0.5 mg/ml

Unconjugated

CD163

CD163 molecule

M130, MM130, SCARI1, scavenger receptor cysteine-rich type 1 protein M130, hemoglobin scavenger receptor, macrophage-associated antigen

Mouse

IgG1

Human

2°C to 8°C

41116161