PathPlus(tm) CD40 Antibody (clone LOB7/6)

LifeSpan BioSciences

PathPlus(tm) CD40 Antibody (clone LOB7/6)

23

Immunohistochemistry: LS-B2596 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2596 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Immunohistochemistry: This product requires protein digestion pre-treatment of paraffin embedded sections prior to staining e. g. pronase or trypsin. Histology Positive Control Tissue: Human Tonsil.. Flo (20 µg/ml), IHC, IHC-Fr (1 - 10 µg/ml), IHC-P (10 µg/ml), IP Immunohistochemistry: LS-B2596 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2596 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Immunohistochemistry: This product requires protein digestion pre-treatment of paraffin embedded sections prior to staining e. g. pronase or trypsin. Histology Positive Control Tissue: Human Tonsil.

Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

LOB7/6

1 mg/ml

Unconjugated

CD40

CD40 molecule

Bp50, CDW40, TNFRSF5, p50, tumor necrosis factor receptor superfamily member 5, B cell surface antigen CD40, B cell-associated molecule, CD40 molecule, TNF receptor superfamily member 5, CD40L receptor

Mouse

IgG2a

Human

2°C to 8°C or -20°C

41116161