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ProductsBack/PathPlus(tm) CORO1A / Coronin 1a Antibody (aa250-300)

PathPlus(tm) CORO1A / Coronin 1a Antibody (aa250-300)

LS-B14784
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetCORO1A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) CORO1A / Coronin 1a Antibody (aa250-300)
  • Delivery Days Customer
    23
  • Application Supplier Note
    IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:100 - 1:2000), IP (2 - 5 µg/mg lysate), WB (1:2000 - 1:10000) IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID11151
  • Target name
    CORO1A
  • Target description
    coronin 1A
  • Target synonyms
    CLABP, CLIPINA, HCORO1, IMD8, TACO, p57, coronin-1A, clipin-A, coronin, actin binding protein, 1A, coronin-1, coronin-like protein A, coronin-like protein p57, tryptophan aspartate-containing coat protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of Coronin 1a/TACO/CORO1A using anti-Coronin 1a/TACO/CORO1A antibody (A04245-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Coronin 1a/TACO/CORO1A antigen affinity purified polyclonal antibody (Catalog # A04245-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Coronin 1a/TACO/CORO1A at approximately 57KD. The expected band size for Coronin 1a/TACO/CORO1A is at 57KD.
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