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ProductsBack/PathPlus(tm) CR1 / CD35 Antibody (clone E11)

PathPlus(tm) CR1 / CD35 Antibody (clone E11)

LS-B7301
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCR1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) CR1 / CD35 Antibody (clone E11)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood.
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo (1:50 - 1:200), IHC, IHC-Fr (1 - 10 µg/ml), IHC-P (10 µg/ml), WB Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    E11
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1378
  • Target name
    CR1
  • Target description
    complement C3b/C4b receptor 1 (Knops blood group)
  • Target synonyms
    C3BR, C4BR, CD35, KN, complement receptor type 1, C3-binding protein, C3b/C4b receptor, CD35 antigen, Knops blood group antigen, complement component (3b/4b) receptor 1 (Knops blood group), complement receptor 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C or -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of CD35 using anti-CD35 antibody (A01022-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human TF1 whole cell lysates, Lane 2: human TF1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD35 antigen affinity purified polyclonal antibody (Catalog # A01022-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD35 at approximately 250 kDa. The expected band size for CD35 is at 224 kDa.
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