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ProductsBack/PathPlus(tm) EMA / MUC1 Antibody (clone ABT-EMA)

PathPlus(tm) EMA / MUC1 Antibody (clone ABT-EMA)

LS-B16147
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetMUC1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) EMA / MUC1 Antibody (clone ABT-EMA)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Use Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH 8.0 Recommended IHC controls tissues are Tonsil, Breast, Pancreas. Resulting localization is Cytoplasmic, Membranous
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC (1:200 - 1:400), IHC-P (1:50) Use Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH 8.0 Recommended IHC controls tissues are Tonsil, Breast, Pancreas. Resulting localization is Cytoplasmic, Membranous
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ABT-EMA
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4582
  • Target name
    MUC1
  • Target description
    mucin 1, cell surface associated
  • Target synonyms
    ADMCKD, ADMCKD1, ADTKD2, CA 15-3, CD227, Ca15-3, EMA, H23AG, KL-6, MAM6, MCD, MCKD, MCKD1, MUC-1, MUC-1/SEC, MUC-1/X, MUC1/ZD, PEM, PEMT, PUM, mucin-1, H23 antigen, breast carcinoma-associated antigen DF3, cancer antigen 15-3, carcinoma-associated mucin, episialin, krebs von den Lungen-6, mucin 1, transmembrane, peanut-reactive urinary mucin, polymorphic epithelial mucin, tumor associated epithelial mucin, tumor-associated epithelial membrane antigen
  • Host
    Mouse
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of MUC1 using anti-MUC1 antibody (A00187-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC1 antigen affinity purified polyclonal antibody (Catalog # A00187-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC1 at approximately 122KD. The expected band size for MUC1 is at 122KD.
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