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ProductsBack/PathPlus(tm) FABP3 / H-FABP Antibody (clone CC68)

PathPlus(tm) FABP3 / H-FABP Antibody (clone CC68)

LS-B6948
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFABP3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) FABP3 / H-FABP Antibody (clone CC68)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (5 µg/ml), WB (1:200)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CC68
  • Conjugate
    Unconjugated
  • Gene ID2170
  • Target name
    FABP3
  • Target description
    fatty acid binding protein 3
  • Target synonyms
    FABP11, H-FABP, M-FABP, MDGI, O-FABP, fatty acid-binding protein, heart, epididymis secretory sperm binding protein, fatty acid binding protein 11, fatty acid binding protein 3, muscle and heart, heart-type fatty acid-binding protein, mammary-derived growth inhibitor, muscle fatty acid-binding protein
  • Host
    Mouse
  • Isotype
    IgG2b
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cardiac FABP using anti-Cardiac FABP antibody (A01734-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac FABP antigen affinity purified polyclonal antibody (Catalog # A01734-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cardiac FABP at approximately 15 kDa. The expected band size for Cardiac FABP is at 15 kDa.
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