PathPlus(tm) HSPB1 / HSP27 Antibody (clone G3.1)

LifeSpan BioSciences

PathPlus(tm) HSPB1 / HSP27 Antibody (clone G3.1)

23

Immunohistochemistry: LS-B383 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B383 was determined to be 1.25 ug/ml.. ELISA (1:10000 - 1:50000), IHC, IHC-P (1.25 µg/ml), WB (1:500 - 1:1000) Immunohistochemistry: LS-B383 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B383 was determined to be 1.25 ug/ml.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

G3.1

Unconjugated

HSPB1

heat shock protein family B (small) member 1

CMT2F, HEL-S-102, HMN2B, HMND3, HS.76067, HSP27, HSP28, Hsp25, SRP27, heat shock protein beta-1, 28 kDa heat shock protein, epididymis secretory protein Li 102, estrogen-regulated 24 kDa protein, heat shock 27 kDa protein, heat shock 27kD protein 1, heat shock 27kDa protein 1, heat shock protein family B member 1, stress-responsive protein 27

Mouse

IgG1

Human

2°C to 8°C or -20°C

41116161