PathPlus(tm) IDH1 / IDH Antibody (aa290-339)

LifeSpan BioSciences

PathPlus(tm) IDH1 / IDH Antibody (aa290-339)

14

Immunohistochemistry: LS-B3573 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3573 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.. IHC, IHC-P (5 µg/ml), WB Immunohistochemistry: LS-B3573 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3573 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

IDH1

isocitrate dehydrogenase (NADP(+)) 1

HEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, PICD, isocitrate dehydrogenase [NADP] cytoplasmic, NADP(+)-specific ICDH, NADP-dependent isocitrate dehydrogenase, cytosolic, NADP-dependent isocitrate dehydrogenase, peroxisomal, epididymis luminal protein 216, epididymis secretory protein Li 26, epididymis secretory sperm binding protein, isocitrate dehydrogenase (NADP(+)) 1, cytosolic, isocitrate dehydrogenase 1 (NADP+), soluble, oxalosuccinate decarboxylase

Rabbit

IgG

Human, Rat

-20°C,2°C to 8°C

41116161