PathPlus(tm) IGF2 Antibody

LifeSpan BioSciences

PathPlus(tm) IGF2 Antibody

23

Human IGF-II

Immunohistochemistry: LS-B7415 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIGF-II (10 ng/ml), a concentration of 3.1-4.6 ug/ml of this antibody is required. ELISA: To detect hIGF-II by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-II (LS-C104384) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-II. Western Blot: To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5-3 ng/lane, under either reducing or non-reducing conditions.. ELISA (0.5 - 2 µg/ml), IHC-P (2.5 - 5 µg/ml), WB (0.1 - 0.2 µg/ml) Immunohistochemistry: LS-B7415 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIGF-II (10 ng/ml), a concentration of 3.1-4.6 ug/ml of this antibody is required. ELISA: To detect hIGF-II by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-II (LS-C104384) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-II. Western Blot: To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

Lyophilized

IGF2

insulin like growth factor 2

C11orf43; GRDF; IGF-II; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor II; insulin-like growth factor type 2; PP9974; preptin; SRS3; T3M-11-derived growth factor

Rabbit

IgG

Human

-20°C,2°C to 8°C,RT

12352203