PathPlus(tm) MAPK3 / ERK1 Antibody (Internal)

LifeSpan BioSciences

PathPlus(tm) MAPK3 / ERK1 Antibody (Internal)

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Human MAPK3 / ERK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MAPK1 (70%).

Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 4 ug/ml.. IHC, IHC-P (10 - 20 µg/ml) Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 4 ug/ml.

ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

...

MAPK3

mitogen-activated protein kinase 3

ERK1; ERK-1; ERT2; extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; insulin-stimulated MAP2 kinase; MAPK 1; microtubule-associated protein 2 kinase; mitogen-activated protein kinase 3; P44ERK1; p44-ERK1; P44MAPK; p44-MAPK; PRKM3

Rabbit

Human, Monkey

-20°C or -80°C,2°C to 8°C

12352203