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ProductsBack/PathPlus(tm) NCAM / CD56 Antibody (clone NCAM1/2217R)

PathPlus(tm) NCAM / CD56 Antibody (clone NCAM1/2217R)

LS-B16307
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetNCAM1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) NCAM / CD56 Antibody (clone NCAM1/2217R)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Immunohistology (Formalin-fixed - 1-2 ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) - Optimal dilution for a specific application should be determined.. IHC-P (1:200) Immunohistology (Formalin-fixed - 1-2 ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) - Optimal dilution for a specific application should be determined.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    NCAM1/2217R
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID4684
  • Target name
    NCAM1
  • Target description
    neural cell adhesion molecule 1
  • Target synonyms
    CD56, MSK39, NCAM, neural cell adhesion molecule 1, antigen recognized by monoclonal antibody 5.1H11, neural cell adhesion molecule, NCAM
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NCAM1 using anti-NCAM1 antibody (A00184-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCAM1 antigen affinity purified polyclonal antibody (Catalog # A00184-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCAM1 at approximately 150KD. The expected band size for NCAM1 is at 150KD.
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